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Does Blue Green Algae Improve Your Stem Cells

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doi: 10.3390/jpm10020049.

The Bluegreen Algae (AFA) Consumption over 48 Hours Increases the Total Number of Peripheral CD34+ Cells in Healthy Patients: Effect of Short-Term and Long-Term Nutritional Supplementation (Curcumin/AFA) on CD34+ Levels (Blood)

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  • PMID: 32521810
  • PMCID: PMC7354690
  • DOI: 10.3390/jpm10020049

Free PMC article

The Bluegreen Algae (AFA) Consumption over 48 Hours Increases the Total Number of Peripheral CD34+ Cells in Healthy Patients: Effect of Short-Term and Long-Term Nutritional Supplementation (Curcumin/AFA) on CD34+ Levels (Blood)

José Joaquín Merino  et al. J Pers Med. .

Free PMC article

Abstract

Several active principles from plants could trigger the release of stem cells from the bone marrow. Stem cell mobilizers have shown side effects in patients. Thus, the purpose of this paper is to find the natural products from plants (curcuminoids, glycosinolate of sulforaphane, AFA bluegreen algae), which could be potential stem mobilizes without adverse side effects. The antioxidant curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-2,5-dione], glycosinolate of sulforaphane (broccoli) or AFA (Aphanizomenon flos) extract promote beneficial effects in patients. The number of circulating stem cells were monitored by HSC marker-CD34 by flow cytometry in peripheral blood from healthy subjects. CD34 is a hematological stem cells (HSC) marker. A double-blind study was conducted in 22 healthy subjects. We have evaluated whether short-term AFA-Aphanizomenon flos aquae-algae or curcuminoids consumption (powder or liquid formulation) over 48 consecutive hours could increase the total number of peripheral CD34+ blood cells (n = 22, n = 5 subjects/group). The total number of circulating CD34+ cells were quantified after short-term and long-term nutritional supplementation; their levels were compared with their own basal levels (n = 5/group, controls: before taking any supplement) or placebo-treated patients (n = 7); their average age was 54 years old. We also evaluated whether long-term nutritional supplementation with several nutraceuticals could enhance HSC mobilization by increasing the total number of peripheral CD-34+ cell after seven or 38 consecutive days of administration (n = 5, with seven placebo-treated patients). The long-term administration take place with these doses/day [curcuminoids: 2000 mg/day, equivalent to 120 mg of curcuminoids/day), glycosinolate of sulforaphane (66 mg/day), plus AFA Algae bluegreen extract (400 mg/day)]. On the last day (10 A.M.) of treatment, blood samples were collected six hours after taking these supplements; the average age was 54 years old. Notably, the blue green AFA algae extract consumption over 48 h enhances HSC mobilization by increasing the total number of peripheral CD34+ cells. The long-term administration with curcuminoids, glycosinolate of sulforaphane, and AFA bluegreen algae extract also increased the total number of CD34-HSC cells after seven or 38 days of consecutive of administration in healthy subjects.

Keywords: AFA (Aphanizomenon flos aquae) algae; chemokines; curcumin; hematopoietic stem cells (HSCs); sulforaphane.

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1

Study Groups.

Figure 2
Figure 2

(protocol). Short-Term AFA Bluegreen Extract or Curcumin Administration over 48 Consecutive Hours with Two Different Formulations (Powder or Liquid Form).

Figure 3
Figure 3

Short-term AFA supplementation over 48 h increases the total number of CD34+ peripheral cells in healthy subjects.

Figure 4
Figure 4

Curcumin treatment did not affect the total number of CD34+ peripheral cells in healthy subjects.

Figure 5
Figure 5

Enhanced HSC mobilization by long-term nutritional supplementation with curcumine curcumin, glycosinolate of sulforaphane in healthy subjects.

Figure 6
Figure 6

Representative flow cytometry for CD34+ peripheral cells (conts) in study groups. (a) Peripheral CD34+ cell (counts) in controls; (b) Peripheral CD34+ cells (counts): short-term AFA consumption over 48 consecutive hours; (c) Total number of peripheral CD34+ in subjects over 48 h of curcumin administration; (d) Effects of long-term nutritional supplementation on total number of CD34+ peripheral cells. Asterisks * are events of data (CD34+ cell counts) within defined regions (R and number) and gates (G and number) for flow cytometry analysis (BD FACSCalibur). The R1*R2*R3 are measured regions for total CD34+ cells (counts) by the BD CellQuest software.

Figure 6
Figure 6

Representative flow cytometry for CD34+ peripheral cells (conts) in study groups. (a) Peripheral CD34+ cell (counts) in controls; (b) Peripheral CD34+ cells (counts): short-term AFA consumption over 48 consecutive hours; (c) Total number of peripheral CD34+ in subjects over 48 h of curcumin administration; (d) Effects of long-term nutritional supplementation on total number of CD34+ peripheral cells. Asterisks * are events of data (CD34+ cell counts) within defined regions (R and number) and gates (G and number) for flow cytometry analysis (BD FACSCalibur). The R1*R2*R3 are measured regions for total CD34+ cells (counts) by the BD CellQuest software.

Figure 6
Figure 6

Representative flow cytometry for CD34+ peripheral cells (conts) in study groups. (a) Peripheral CD34+ cell (counts) in controls; (b) Peripheral CD34+ cells (counts): short-term AFA consumption over 48 consecutive hours; (c) Total number of peripheral CD34+ in subjects over 48 h of curcumin administration; (d) Effects of long-term nutritional supplementation on total number of CD34+ peripheral cells. Asterisks * are events of data (CD34+ cell counts) within defined regions (R and number) and gates (G and number) for flow cytometry analysis (BD FACSCalibur). The R1*R2*R3 are measured regions for total CD34+ cells (counts) by the BD CellQuest software.

Figure 6
Figure 6

Representative flow cytometry for CD34+ peripheral cells (conts) in study groups. (a) Peripheral CD34+ cell (counts) in controls; (b) Peripheral CD34+ cells (counts): short-term AFA consumption over 48 consecutive hours; (c) Total number of peripheral CD34+ in subjects over 48 h of curcumin administration; (d) Effects of long-term nutritional supplementation on total number of CD34+ peripheral cells. Asterisks * are events of data (CD34+ cell counts) within defined regions (R and number) and gates (G and number) for flow cytometry analysis (BD FACSCalibur). The R1*R2*R3 are measured regions for total CD34+ cells (counts) by the BD CellQuest software.

Figure 7
Figure 7

Gate definitions for BD Cell Quest software.

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